Interaction mechanism between Cd2+ ions and DNA from the kidney of the silver crucian carp |
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Authors: | Fashui Hong Cheng Wu Chao Liu Kang Wu Fengqing Gao Fan Yang |
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Institution: | (1) College of Life Sciences, Suzhou University, 215006 Suzhou, People's Republic of China |
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Abstract: | Cadmium is one of the most toxic heavy metals and is known to accumulate in freshwater food chains. The underlying mechanism
for its genotoxicity has not been investigated for any freshwater fish. It has, however, been suggested that cadmium-induced
carcinogenesis might involve either direct or indirect interaction of Cd2+ with DNA. The interaction between Cd2+ and DNA from the kidney of the silver crucian carp (Carassius auratus gibelio) in vitro and in vivo is investigated by spectrophotometric methods and agarose gel electrophoresis methods. Cd2+ could insert into DNA basepairs, bind to nucleic acid, and result in notable hypochromicities. The analysis of agarose gel
electrophoresis, proves that Cd2+ at different concentrations does not cause DNA cleavage in vitro; however, kidneys display the classical laddering degradation
of DNA in vivo, which is the result of the promotion of deoxyribonuclease activity or inhibition of superoxide dismutase and
catalyse activity and the accumulation of reactive oxygen species caused by Cd2+ ions in vivo. |
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Keywords: | Cadmium silver crucian carp DNA absorption damage gel electrophoresis DNase activity antioxidant enzyme activity ROS |
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