Characterization of <Emphasis Type="Italic">Rubus fruticosus</Emphasis> mitochondria and salicylic acid inhibition of reactive oxygen species generation at Complex III/Q cycle: potential implications for hypersensitive response in plants |
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Authors: | Wagner Rodrigo de Souza Ricardo Vessecchi Daniel Junqueira Dorta Sérgio Akira Uyemura Carlos Curti Carem Gledes Vargas-Rechia |
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Institution: | 1.Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeir?o Preto,Universidade de S?o Paulo,Ribeir?o Preto,Brazil;2.Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeir?o Preto,Universidade de S?o Paulo,Ribeir?o Preto,Brazil;3.Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeir?o Preto,Universidade de S?o Paulo,Ribeir?o Preto,Brazil |
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Abstract: | In addition to adenosine triphosphate (ATP) production, mitochondria have been implicated in the regulation of several physiological
responses in plants, such as programmed cell death (PCD) activation. Salicylic acid (SA) and reactive oxygen species (ROS)
are essential signaling molecules involved in such physiological responses; however, the mechanisms by which they act remain
unknown. In non-photosynthesizing tissues, mitochondria appear to serve as the main source of ROS generation. Evidence suggests
that SA and ROS could regulate plant PCD through a synergistic mechanism that involves mitochondria. Herein, we isolate and
characterize the mitochondria from non-photosynthesizing cell suspension cultures of Rubus fruticosus. Furthermore, we assess the primary site of ROS generation and the effects of SA on isolated organelles. Mitochondrial Complex
III was found to be the major source of ROS generation in this model. In addition, we discovered that SA inhibits the electron
transport chain by inactivating the semiquinone radical during the Q cycle. Computational analyses confirmed the experimental
data, and a mechanism for this action is proposed. |
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