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实时荧光定量PCR技术及其应用
引用本文:李文涛,王俊东,杨利峰,王宏伟,韩少杰. 实时荧光定量PCR技术及其应用[J]. 生物技术通讯, 2006, 17(1): 112-114
作者姓名:李文涛  王俊东  杨利峰  王宏伟  韩少杰
作者单位:山西农业大学动物科技学院,山西,太谷,030801
基金项目:李文涛(1979-),男,硕士.
摘    要:定量PCR的问世是继定性PCR(即常规PCR)后分子生物学方法学研究的一大飞跃,实现了对核酸信息量的分析比较,为疾病的诊断和治疗提供了更多、更有效的基因水平信息。本文介绍实时荧光定量PCR技术及其在兽医领域中的应用,包括近年来研究和应用较多的几种定量PCR技术。

关 键 词:定量PCR  基因  应用
文章编号:1009-0002(2006)01-0112-03
收稿时间:2005-05-16
修稿时间:2005-05-16

Real-Time Fluorescent Quantitative PCR Technique and its Application
LI Wen-tao,WANG Jun-dong,YANG Li-feng,WANG Hong-wei,HAN Shao-jie. Real-Time Fluorescent Quantitative PCR Technique and its Application[J]. Letters in Biotechnology, 2006, 17(1): 112-114
Authors:LI Wen-tao  WANG Jun-dong  YANG Li-feng  WANG Hong-wei  HAN Shao-jie
Abstract:The emergence of quantitative polymerase chain reaction PCR after the traditional PCR is a large progress in the method on molecular biology. It can be used to analysis the quantity of information of nucleic acid and the more effective information on gene levels can be provided for disease diagnosis and therapy. The theory and methods of the real-time fluorescent quantitative PCR FQ-PCR and it's application have been introduced in this paper including some traditional quantitative polymerase chain reaction techniques.
Keywords:quantitative PCR gene application
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