Selecting genetic transformants of indica and indica-derived rice cultivars using bispyribac sodium and a mutated ALS gene |
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Authors: | Yojiro Taniguchi Motoshige Kawata Ikuo Ando Tsutomu Shimizu Masahiro Ohshima |
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Institution: | (1) National Institute of Crop Science, Kannondai 2-1-18, Tsukuba Ibaraki, 305-8518, Japan;(2) Life Science Research Institute, Kumiai Chemical Industry Co. Ltd, 3360 Kamo, Kikugawa Shizuoka, 439-0031, Japan; |
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Abstract: | Bispyribac sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of acetolactate synthase (ALS) activity.
ALS is an enzyme in the biosynthetic pathway for branched-chain amino acids. A mutant form of rice ALS (OsmALS W548L/S627I]) that confers resistance to BS can be used as an in vitro selection marker gene for plant transformation. Since indica and indica-derived cultivars are thought to have lower BS sensitivity than japonica rice, the application of BS as a selectable reagent for genetic transformation in indica and indica-derived cultivars is more challenging than for japonica cultivars. In this study, callus and seedlings of eight different rice cultivars (five indica-derived cultivars, two indica cultivars and one japonica cultivar) were tested for BS sensitivity. Our study indicates for the first time that callus shows a higher sensitivity to
BS than seedlings in indica and indica-derived cultivars. We used BS with OsmALS W548L/S627I] to select transformed calli, and transgenic rice plants from indica and indica-derived cultivars were successfully obtained. |
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