Contribution of hydrolyzed nucleic acids and their constituents to the apparent amino acid composition of biological compounds. |
| |
Authors: | G V Paddock G B Wilson A C Wang |
| |
Institution: | Department of Basic and Clinical Immunology and Microbiology, Medical University of South Carolina, Charleston, South Carolina 29403 USA |
| |
Abstract: | Acid hydrolysis of protein-free mixtures of nucleotides, nucleosides, and nucleic acids yields amino acids, free bases, and possibly other unidentified fragments when analyzed by thin-layer chromatography and by standard amino acid analysis. Glycine is the predominant amino acid detected, which may constitute 47–97% of the apparent amino acid composition, depending on the type of material subjected to hydrolysis. Obviously, hydrolyzed nucleic acids or their constituents can therefore contribute to the apparent amino acid composition of a supposedly pure peptide or of other more complex mixtures of compounds mistakenly believed to contain only protein. To circumvent this problem, we suggest that nucleotides or nucleic acid moieties should be removed from any product for which the amino acid composition is desired, and that whenever a large glycine peak is noted in a hydrolyzed sample, the presence of nucleic acids or their constituents should be suspected. |
| |
Keywords: | Correspondence and reprint requests should be addressed to Dr G B Wilson |
本文献已被 ScienceDirect 等数据库收录! |
|