Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil |
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Authors: | de Filippis Ivano de Lemos Ana Paula S Hostetler Jessica B Wollenberg Kurt Sacchi Claudio T Dunning Hotopp Julie C Harrison Lee H Bash Margaret C Prevots D Rebecca |
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Affiliation: | National Quality Control Institute (INCQS), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil. |
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Abstract: | BackgroundNeisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sample of clinical disease isolates from 2004, and a temporally representative sample for the state of São Paulo (1988–2006) for study (n = 372).MethodsWe performed multi-locus sequence typing (MLST) and sequence analysis of five outer membrane protein (OMP) genes, including novel vaccine targets fHbp and nadA.ResultsIn 2004, strain B:4:P1.15,19 clonal complex ST-32/ET-5 (cc32) predominated throughout Brazil; regional variation in MLST sequence type (ST), fetA, and porB was significant but diversity was limited for nadA and fHbp. Between 1988 and 1996, the São Paulo isolates shifted from clonal complex ST-41/44/Lineage 3 (cc41/44) to cc32. OMP variation was associated with but not predicted by cc or ST. Overall, fHbp variant 1/subfamily B was present in 80% of isolates and showed little diversity. The majority of nadA were similar to reference allele 1.ConclusionsA predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp. |
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