Transient expression of AtNCED3 and AAO3 genes in guard cells causes stomatal closure in Vicia faba |
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Authors: | Vanessa Melhorn Kunimi Matsumi Hanae Koiwai Keiichi Ikegami Masanori Okamoto Eiji Nambara Florian Bittner Tomokazu Koshiba |
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Affiliation: | (1) Department of Biological Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan;(2) Department of Plant Biology, Technical University of Braunschweig, Braunschweig, Germany;(3) Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Japan;(4) Growth Regulation Research Group, RIKEN Plant Science Center, Yokohama, Japan;(5) Present address: Plant Functional Genomics Research Group, RIKEN Plant Science Center, Yokohama, Japan |
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Abstract: | Abscisic acid (ABA) regulates stomatal closure in response to water loss. Here, we examined the competence of guard cells to synthesize ABA, using two Arabidopsis ABA biosynthetic enzymes. 35S pro::AtNCED3-GFP and AAO3-GFP were introduced into guard cells of broad bean leaves. AtNCED3-GFP expression was detected at the chloroplasts, whereas green fluorescent protein (GFP) and AAO3-GFP were in the cytosol. The stomatal aperture was decreased in AtNCED3-GFP- and AAO3-GFP-transformed guard cells. This indicated that ABA biosynthesis is stimulated by heterologous expression of AtNCED3 and Arabidopsis aldehyde oxidase 3 (AAO3) proteins, which both seem to be regulatory enzymes for ABA biosynthesis in these cells. Furthermore, stomatal closure by the expression of AtNCED3 and AAO3 suggested that the substrates of the enzymes are present and native ABA-biosynthesis enzymes are active in guard cells. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. V. Melhorn and K. Matsumi contributed equally to this work. |
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Keywords: | Abscisic acid Arabidopsis aldehyde oxidase 3 (AAO3) Broad bean 9-cis-epoxycarotenoid dioxygenase (NCED) Stomatal closure Transient expression |
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