| 1,3-丙二醇产生茵基因组重排育种 |
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| 引用本文： | 卢圣国,李霜,罗芳,祝扬扬,熊俊,孟庆雄. 1,3-丙二醇产生茵基因组重排育种[J]. 微生物学报, 2011, 51(4): 474-479 |
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| 作者姓名： | 卢圣国 李霜 罗芳 祝扬扬 熊俊 孟庆雄 |
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| 作者单位： | 1. 昆明理工大学生命与科学技术学院,昆明,650216
2. 南京工业大学国家生化中心,南京,211816 |
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| 摘 要： | [目的]本文以肺炎克雷伯氏杆菌为研究对象,利用基因组重排技术,提高其对发酵体系中主要产物的耐受性,获得1,3-丙二醇高产菌.[方法]以含预处理后的出发菌株的补料发酵终点液的96孔板为筛选方法,利用基因组重排技术育种.[结果]筛选到的5株高产菌株(LSG1,LSG2,LSG4,LSG5,LSG6),在3升罐批次发酵中的1...
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| 关 键 词： | 1,3-丙二醇 肺炎克雷伯氏杆菌 基因组重排 原生质体 补料发酵终点液 |
| 收稿时间： | 2010-11-11 |
| 修稿时间： | 2011-01-02 |
Screening and breeding high 1,3-propanediol producing strains by genome shuffling |
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| Shengguo Lu,Shuang Li,Fang Luo,Yangyang Zhu,Jun Xiong and Qingxiong Meng. Screening and breeding high 1,3-propanediol producing strains by genome shuffling[J]. Acta microbiologica Sinica, 2011, 51(4): 474-479 |
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| Authors: | Shengguo Lu Shuang Li Fang Luo Yangyang Zhu Jun Xiong Qingxiong Meng |
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| Affiliation: | Faculty of Life science and technology, Kunming university of science and technology, Kunming 650216, China;National bio-chemical engineering technique research center, Nanjing university of technology, Nanjing 211816, China;National bio-chemical engineering technique research center, Nanjing university of technology, Nanjing 211816, China;Faculty of Life science and technology, Kunming university of science and technology, Kunming 650216, China;Faculty of Life science and technology, Kunming university of science and technology, Kunming 650216, China;Faculty of Life science and technology, Kunming university of science and technology, Kunming 650216, China |
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| Abstract: | [Objective]To improve the tolerance of main metabolites,we used genome shuffling to achieve high 1,3-propanediol producing mutants.[Methods]Based on 96 deep-well palates containing prepared ended fed-batch broth as an efficient selection method,genome shuffling has been applied in strain improvement.[Results]Five high producers were obtained after genome shuffling(LSG1,LSG2,LSG4,LSG5 and LSG6).During batch fermentation(3 L),the 1,3-propanediol production of the five mutants were improved 17.0%,19.0%,12.9%,2... |
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| Keywords: | Keywords: 1 3-propanediol Klebsiella pneumoniae genome shuffling |
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