A closed system for the filtration of Mycobacterium bovis liquid cultures using disposable capsule filters |
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Authors: | E A Sugden P Martineau E B Rohonczy & K Stilwell |
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Institution: | Agriculture and Agri-Food Canada, Animal Diseases Research Institute, Nepean, Ontario, Canada |
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Abstract: | A filtration system was designed to sterilize large volumes of Mycobacterium bovis BCG Tokyo culture safely, needed to purify protein antigens for immunodiagnosis of bovine tuberculosis. A closed system consists of culture bottles connected to three disposable filter capsules of decreasing pore size in series : a depth prefilter over a 1·2 μm filter ; a 0·8 μm prefilter over a 0·45 μm filter ; and a 0·2 μm sterile filter. Low air pressure (3 psi) forces liquid from below the bacillary pellicle. The system features a stainless steel clamp to hold rubber stoppers on the culture bottles, pleated filters to exclude bacillary clumps, a quick disconnector to minimize aerosols, and a closed system with plastic disposable filters that can be autoclaved as a unit without dismantling. |
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