Linker histone H1 stimulates DNA strand exchange between short oligonucleotides retaining high sensitivity to heterology |
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Authors: | Bocharova Tatiana N Smirnova Elena A Volodin Alexander A |
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Affiliation: | Institute of Molecular Genetics of the Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russia. |
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Abstract: | The interaction of human linker histone H1(0) with short oligonucleotides was characterized. The capability of the histone to promote DNA strand exchange in this system has been demonstrated. The reaction is reversible at saturating amounts of H1 corresponding to complete binding of the oligonucleotide substrates with the histone. In our conditions the complete saturation of DNA with the histone occurs at a ratio of one protein molecule per about 60 nucleotides irrespectively of DNA strandedness. In contrast to the DNA strand exchange promoted by RecA-like enzymes of homologous recombination the H1 promoted reaction exhibits low tolerance to interruptions of homology between oligonucleotide substrates comparable to those for the case of spontaneous strand exchange between free DNA molecules at elevated temperatures and the exchange promoted by some synthetic polycations. |
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Keywords: | linker histone–oligonucleotide binding DNA strand exchange sensitivity to heterology |
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