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Selection of an extracellular esterase-producing microorganism |
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| Authors: | Guillermo R Castro Adrián O Stettler Marcela A Ferrero Faustino Siñeriz |
| Institution: | (1) Depto. de Qca. Biológica, Fac. de Ciencias Exactas y Naturales, Univ. de Buenos Aires. Pab. II-Cdad. Universitaria (Nuñez), Buenos Aires, Argentina;(2) Cátedra de Microbiología Superior, Facultad de Quimica, Bioquímica y Farmacia, Univ. Nac. de Tucumán-PROIMI-MIRCEN, Avda. Belgrano y Pje, Caseros, 4000 Tucumán, Argentina |
| Abstract: | Summary The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to theBacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. |
| Keywords: | Esterase screening test Esterase production B subtilis esterase Temperature profile pH profile |
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