Quantification of ethylene losses in different container-seal systems and comparison of biotic and abiotic contributions to ethylene accumulation in cultured tissues |
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Authors: | A Mensuali-Sodi M Panizza F Tognoni |
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Institution: | Scuola Superiore Studi Universitari e Perfezionamento, S. Anna, Via Carducci 40, 1-56100 pisa, Italy;Dipartimento di Biologia delle piante Agraie, Univ. of pisa, Viale delle piagge 23, I-56100 pisa, Italy. |
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Abstract: | Ethylene losses and release were compared in four container- seal systems for in vitro cultures: Erlenmeyer flasks sealed with silicone, caoutchouc or styrene butadiene stoppers and glass bottles with screw caps supplied with caoutchouc septa. The last systems Proved to be the most suitable (i. e. minimum ethylene loss and release) to determine ethylene accumulation during axillary budding of lavandin ( Lavandula officinalis Chaix × Lavandula latifolia Villars cv. Grosso). Gelling agents (agar and Gelrite) also discharges ethylene and agar was identified as the main abiotic source. Mathematical elaboration of experimental data was then performed to estimate biological ethylene production. |
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Keywords: | Ethlylene Gelrite lavandin Lavandula rubber sealing system tissue culture |
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