Species-dependent differences of the biochemical properties of diamine oxidase |
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| Authors: | J C Romijn C F Verkoelen T A Splinter |
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| Affiliation: | 2. Department of Neurology, Brain Attack Center Ota Memorial Hospital, Fukuyama, Japan;3. Department of Cardiology, Brain Attack Center Ota Memorial Hospital, Fukuyama, Japan |
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| Abstract: | Diamine oxidase (DAO) from tissues of mice, rats and humans showed different properties with respect to stability and kinetic parameters. DAO-activities in homogenates of rat or human tissues, but not of mouse tissues, rapidly decreased upon storage at -20 degrees C. The Km-value for putrescine was 90 microM in mouse kidney or intestine. In rats different Km-values were observed before (272 microM) and after freezing (102 microM). A similar effect was observed with DAO in human kidney (321 and 39 microM, respectively). Treatment of rats with heparin resulted in a depletion of intestinal DAO and the concomitant appearance of DAO in blood. The enzyme remaining in the intestine showed the lower Km-value. |
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