Multiple Ty-mediated chromosomal translocations lead to karyotype changes in a wine strain of Saccharomyces cerevisiae. |
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Authors: | N Rachidi P Barre and B Blondin |
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Institution: | (1) Laboratoire de Microbiologie et Technologie des Fermentations, IPV, INRA-ENSA, 2 Place Viala, F-34060 Montpellier Cedex, France e-mail: blondin@ensam.inra.fr Tel.: +33-49-9612542; Fax: +33-49-9612857, FR |
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Abstract: | Enological strains of Saccharomyces cerevisiae display a high level of chromosome length polymorphism, but the molecular basis of this phenomenon has not yet been clearly
defined. In order to gain further insight into the molecular mechanisms responsible for the karyotypic variability, we examined
the chromosomal constitution of a strain known to possess aberrant chromosomes. Our data revealed that the strain carries
four rearranged chromosomes resulting from two reciprocal translocations between chromosomes III and I, and chromosomes III
and VII. The sizes of the chromosomal fragments exchanged through translocation range from 40 to 150 kb. Characterization
of the breakpoints indicated that the translocations involved the RAHS of chromosome III, a transposition hot-spot on the
right arm of chromosome I and a region on the left arm of chromosome VII. An analysis of the junctions showed that in all
cases Ty elements were present and suggested that the translocations result from recombination between transposable Ty elements.
The evidence for multiple translocations mediated by Ty elements in a single strain suggests that spontaneous Ty-driven rearrangement
could be quite common and may play a major role in the alteration of karyotypes in natural and industrial yeasts.
Received: 18 December 1998 / Accepted: 26 March 1999 |
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Keywords: | Wine yeast Chromosome length polymorphism Chromosomal rearrangements Ty elements Genome evolution |
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