Circadian clock genes period and cycle regulate photoperiodic diapause in the bean bug Riptortus pedestris males |
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Authors: | Ikeno Tomoko Numata Hideharu Goto Shin G |
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Affiliation: | a Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi, Osaka 558-8585, Japan b Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan |
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Abstract: | The photoperiodic response is crucial for many insects to adapt to seasonal changes in temperate regions. It was recently shown that the circadian clock genes period (per) and cycle (cyc) are involved in the photoperiodic regulation of reproductive diapause in the bean bug Riptortus pedestris females. Here, we investigated the involvement of per and cyc both in the circadian rhythm of cuticle deposition and in the photoperiodic diapause of R. pedestris males using RNA interference (RNAi). RNAi of per and cyc disrupted the cuticle deposition rhythm and resulted in distinct cuticle layers. RNAi of per induced development of the male reproductive organs even under diapause-inducing short-day conditions, whereas RNAi of cyc suppressed development of the reproductive organs even under diapause-averting long-day conditions. Thus, the present study suggests that the circadian clock operated by per and cyc governs photoperiodism of males as that of females. |
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Keywords: | CLK, CLOCK CYC, CYCLE per, period tim, timeless RNAi, RNA interference dsRNA, double-stranded RNA bla, β-lactamase ZT, Zeitgeber time |
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