| 人红细胞生成素的分离、纯化及鉴定 |
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| 引用本文： | 施秉仪,刘福陆,周淑珍,吴祖泽.人红细胞生成素的分离、纯化及鉴定[J].中国生物化学与分子生物学报,1987,3(4):351-358. |
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| 作者姓名： | 施秉仪 刘福陆 周淑珍 吴祖泽 |
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| 作者单位： | 军事医学科学院放射医学所，军事医学科学院放射医学所，军事医学科学院放射医学所，军事医学科学院放射医学所 北京 卫生部北京老年医学所生化室，北京，北京，北京 |
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| 摘 要： | 用自制的苯基-琼脂糖CL-4B和羟基邻灰石等层析材料,从再生障碍性贫血病人尿中分离、纯化制得了红细胞生成素(EPO)。用多血小鼠红细胞~(56)Fe参入法测定该制品在体内的生物活力。用小鼠与人骨髓红系祖细胞培养法测其在体外的生物活力。实验结果说明,我们自制的EPO制品,不仅能用于动物,也能用于人骨贿红系祖细胞的培养。用Azocoll法测该制品中蛋白水解酶活力为阴性。
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| 关 键 词： | 红细胞生成素 苯基一琼脂糖CL-4B 羟基邻灰石 |
| 收稿时间： | 1987-08-20 |
THE ISOLATION, PURIFICATION AND IDENTIFICATION OF HUMAN ERYTHROPOIETIN |
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| She,Bing-yi Lui,Fu-lu Zhou,Shu-zhen Wu,Chu-tse.THE ISOLATION, PURIFICATION AND IDENTIFICATION OF HUMAN ERYTHROPOIETIN[J].Chinese Journal of Biochemistry and Molecular Biology,1987,3(4):351-358. |
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| Authors: | She Bing-yi Lui Fu-lu Zhou Shu-zhen Wu Chu-tse |
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| Institution: | (Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing |
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| Abstract: | Human erythropoietin(EPO)derived from urine of patients with aplastic anemia had been isolated and purified by chromatography on column filled with self prepared phenyl-Sepharose CL-4B and hydroxylapatite as support.The biological activity of the purified EPO was assayed in vivo by measuring its stimulation of 59Fe incorporation into the circulating red cells of the hypoxic polycythemic mouse. The in vitro stimulated effect was assayed with mouse fetal liver, bone marrow and human bone marrow erythroid cells in culture. Experimental evidences showed that our preparation could be used in both animal and human bone marrow erythroid cells culture.No protease activity was detected in the EPO preparation by Azocoll assay. |
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| Keywords: | erythropoietin(EPO) Pheny-sepharose CL-1B Hydroxylapatite |
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