Substrate interactions in dehalogenation of 1,2-dichloroethane, 1,2-dichloropropane,and 1,1,2-trichloroethane mixtures by Dehalogenimonas spp. |
| |
Authors: | Jacob L Dillehay Kimberly S Bowman Jun Yan Fred A Rainey William M Moe |
| |
Institution: | 1. Department of Civil and Environmental Engineering, Louisiana State University, 3515B Patrick Taylor Hall, Baton Rouge, LA, 70803, USA 2. Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA 4. Department of Microbiology and Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, USA 3. Department of Biological Sciences, University of Alaska Anchorage, Anchorage, AK, 99508, USA
|
| |
Abstract: | When chlorinated alkanes are present as soil or groundwater pollutants, they often occur in mixtures. This study evaluated substrate interactions during the anaerobic reductive dehalogenation of chlorinated alkanes by the type strains of two Dehalogenimonas species, D. lykanthroporepellens and D. alkenigignens. Four contaminant mixtures comprised of combinations of the chlorinated solvents 1,2-dichloroethane (1,2-DCA), 1,2-dichloropropane (1,2-DCP), and 1,1,2-trichloroethane (1,1,2-TCA) were assessed for each species. Chlorinated solvent depletion and daughter product formation determined as a function of time following inoculation into anaerobic media revealed preferential dechlorination of 1,1,2-TCA over both 1,2-DCA and 1,2-DCP for both species. 1,2-DCA in particular was not dechlorinated until 1,1,2-TCA reached low concentrations. In contrast, both species concurrently dechlorinated 1,2-DCA and 1,2-DCP over a comparably large concentration range. This is the first report of substrate interactions during chlorinated alkane dehalogenation by pure cultures, and the results provide insights into the chlorinated alkane transformation processes that may be expected for contaminant mixtures in environments where Dehalogenimonas spp. are present. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|