Methanol determination in Pichia pastoris cultures by flow injection analysis |
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Authors: | C. Almuzara O. Cos M. Baeza D. Gabriel F. Valero |
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Affiliation: | (1) Escola Universitària Politècnica del Medi Ambient, 08100 Mollet del Vallés, Spain;(2) Departament d'Enginyeria Química, ETSE, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain |
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Abstract: | An automated reverse flow injection analysis (r-FIA) system using stop-flow technique for quantifying methanol based on the enzymatic reactions of alcohol oxidase and peroxidase was developed. The system permitted methanol analysis in a linear range of 0.006-0.1 g methanol l–1 without external dilution, and with a sampling frequency of 12 analyses per hour, with a relative standard deviation of 1.16%. The analyser was validated analysing samples from a Pichia pastoris fermentation producing a heterologous protein. |
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Keywords: | alcohol oxidase flow injection analysis methanol peroxidase Pichia pastoris |
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