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Localization of beta-endorphins in the rat spinal cord using avidin-biotin technology |
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| Authors: | P L De Riu A Bitti S Rubino G Virdis P L Fiori |
| Abstract: | The avidin-biotin-peroxidase technique is proposed for the immunological localization of beta-endorphin in the rat spinal cord. A rabbit specific antibody anti-human beta-endorphin was first obtained and then identified by immunoblotting and incubated with a quick-frozen section of young rat spinal cord. The use of a specific antibody with the immunoperoxidase reaction gave a morphological visualization of the beta-endorphin in the histological sections of the rat spinal cord. |
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