Homotropic effect of CO 2 in ribulose-1,5-diphosphate carboxylase reaction |
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Authors: | T Murai T Akazawa |
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Institution: | Research Institute for Biochemical Regulation School of Agriculture, Nagoya University Chikusa, Nagoya, Japan |
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Abstract: | The concentration effect of aqueous CO2 on the reaction velocity of spinach leaf ribulose-1,5-diphosphate carboxylase has been reevaluated. The homotropic effect of CO2 in the enzyme reaction supports the previously reported allosteric nature of the enzyme in the CO2-fixation process in chloroplasts. The concentration of CO2 giving the half maximal reaction velocity, S0.5, has been calculated to be 1.47 × 10−5M. |
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