Enzyme studies on human and snail beta-mannosidase using a fluorescence assay and an HPLC/diode array method with Man beta (1-4)GlcNAc as substrate. |
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| Authors: | J van Pelt C H Hokke L Dorland M Duran |
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| Institution: | University Children's Hospital, Het Wilhelmina Kinderziekenhuis, Utrecht, The Netherlands. |
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| Abstract: | 1. Snail beta-mannosidase showed a Km value of 0.05 mM toward MU-beta-Man and could not be inhibited by Man, GlcNAc, Man beta(1-4)GlcNAc, Man beta(1-4)GlcNAc beta(1-N)urea or Man beta(1-4) GlcNAc beta(1-4)GlcNAc. 2. The Km value of the snail enzyme towards Man beta(1-4)GlcNAc, as measured by HPLC, was 10 mM, explaining the lack of inhibition. 3. The Km value of the human serum beta-mannosidase towards MU-beta-Man was 0.3 mM, but the human enzyme was not capable of degrading Man beta(1-4)GlcNAc in detectable amounts. |
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