Facilitated isolation of rare recombinants by ligase chain reaction: selection for intragenic crossover events in the Drosophila optomotor-blind gene |
| |
Authors: | Jü rgen Balles Gert O. Pflugfelder |
| |
Affiliation: | (1) Theodor-Boveri-Institut, Biozentrum, Lehrstuhl für Genetik, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany |
| |
Abstract: | Ligase chain reaction (LCR) was evaluated as a tool for the detection of point mutations. For the mutation studied, the specificity of the method is sufficient to detect the mutant allele in the presence of a 200-fold molar excess of the wild-type sequence. LCR was therefore employed in a genetic recombination experiment as a probe for a recessive lethal point mutation. LCR greatly facilitated the isolation of a rare recombinant originating from a crossover event in the 40 kb interval separating the lethal mutation and an enhancer trap insertion in the optomotor-blind locus. The recombinant will allow the study of gene control in situ, in a largely unperturbed regulatory environment. |
| |
Keywords: | Ligase chain reaction Intragenic recombination Gene regulation Point mutation Drosophila melanogaster |
本文献已被 SpringerLink 等数据库收录! |