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Effect of mannan oligosaccharide elicitor and ferulic acid on enhancement of laccases production in liquid cultures of basidiomycetes |
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| Authors: | Sophie Vanhulle Romeo Radman Roberto Parra Tingting Cui Christian-Marie Bols Thierry Tron Giovanni Sannia Tajalli Keshavarz |
| Affiliation: | aCatholic University of Louvain, Louvain-la-Neuve, Belgium bDepartment of Applied and Molecular Biosciences, University of Westminster, London, United Kingdom cWetlands Engineering SPRL, Louvain-la-Neuve, Belgium dLaboratoire de Bioinorganique Structurale Marseille, France eDepartment of Organic Chemistry and Biochemistry, University of Naples, Naples, Italy |
| Abstract: | The effects of mannan oligosaccharides preparation (MO), as elicitor, and ferulic acid inducer for enhancement in laccases production in liquid cultures of three strains of basidiomycetes, Pycnoporus sanguineus, Coriolopsis polyzona and Pleurotus ostreatus was studied using a full factorial 32 experimental design. MO, either individually or combined with ferulic acid, enhanced laccases levels in the three different strains of the white-rot fungi. The enhancement of laccases production was species specific with the highest increase in liquid cultures of P. sanguineus (88-fold) followed by P. ostreatus (3-fold) and C. polyzona (2-fold). Separate additions of 75 mg/l of MO to the cultures of P. sanguineus and P. ostreatus caused the increase while a combined addition of 150 mg/l of MO and 1 mM ferulic acid resulted in the optimal production of laccases in the cultures of C. polyzona. |
| Keywords: | Basidiomycetes Mannan oligosaccharides Ferulic acid Laccases Response surface methodology |
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