Impairment of human polymorphonuclear leukocyte chemotaxis by 2,5-hexanedione |
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Authors: | Mario Governa Matted Valentino Isa Visona' Marsilia Rocco |
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Affiliation: | (1) Institute of Occupational Medicine, University of Ancona, Ancona, Italy;(2) Clinica di Medicina del Lavoro, Nuovo Ospedale Regionale, 60020, Tourrette di Ancona, Italia |
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Abstract: | The effect of n-hexane metabolites on human polymorphonuclear leukocyte chemotaxis and luminol-dependent chemiluminescence was investigated. No effect was detected when 2-hexanol, 2-hexanone and -valerolactone were used, 2,5-hexanedione at 75 g/ml inhibited chemotaxis and a direct correlation between increasing the xenobiotic concentration and the degree of inhibition was found. Chemotactic peptide-induced chemiluminescence was not affected by 2,5-hexanedione. In order to clarify the phenomenon, plasma membrane fluidity was investigated by fluorescence polarization of the fluorescent probe trimethylammonium diphenylhexatriene. 2,5-hexanedione increased the membrane fluidity, while the other n-hexane metabolites did not change the degree of flourescence polarization. Results suggest that the cellular functions modulated by membrane-cytoskeletal organization are affected by 2,5-hexanedione also at the low concentrations.Abbreviations 2,5-Hxdn 2,5-hexanedione - 2-Hxl 2-hexanol - 2-Hxn 2-hexanone - PMN polymorphonuclear leukocyte - TMA DPH trimethylammonium diphenylhexatriene - -V1 -valerolactone |
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Keywords: | granulocyte chemotaxis n-hexane metabolites 2,5-hexanedione |
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