Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells |
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| Authors: | Du Wen Yanhong Xue Kuo Liang Tianyi Yuan Jingze Lu Wei Zhao Tao Xu Liangyi Chen |
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| Institution: | 1. National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2. Lab of Cell Secretion and Metabolism, Institute of Molecular Medicine, Peking University and National Center for Nanoscience and Technology, Beijing, 100871, China; 3. Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China; 4. Graduate School of the Chinese Academy of Sciences, Beijing 100864, China |
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| Abstract: | Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the molecular mechanism and physiological function of bulk endocytosis remain elusive. In pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca2+ entry and suppressed by the inhibition of dynamin function. Moreover, defects in bulklike endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice, which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability. |
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| Keywords: | bulk-like endocytosis clathrin-independent endocytosis dynamin diabetic KKAy mice |
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