Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola (Strophariaceae) |
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| Authors: | CHEN Wei-Min- CHAI Hong-Mei- ZHANG Xiao-Lei- LI Shu-Hong- ZHAO Yong-Chang- |
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| Affiliation: | 1.Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650221, China; 2.Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming 650221, China;3.Key Lab of Southwestern ;Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650221, China |
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| Abstract: | A 4231bp DNA fragment of B mating type pheromone receptor from strain YAASM0711 of Agrocybe salicacola was obtained by using degenerate PCR and DNA walking techniques. The result of alignment and structure prediction of DNA sequences showed that one 1194bp nucleotides gene ASRcb1 encoding B mating pheromone receptor was found, including four introns(72bp, 49bp, 48bp and 41bp) and five extrons (217bp, 113bp, 67bp, 138bp and 449bp). The spliced open reading frame (ORF) contains 984bp nucleotides encoding 327 amino acid residues, and includes seven transmembrane protein regions as in its similar sequences of Coprinus cinerea and Laccaria bicolor pheromone receptors. The genetic evolution of pheromone receptor showed ASRcb1 was clustered with more receptors, which suggested multiple ways of evolution occurred in fungi. |
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| Keywords: | Agrocybe salicacola Pheromone receptor Mating type Degenerate PCR Amino acid sequence |
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