Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA |
| |
| Authors: | Wei Li Xinlu Wang Guangxia Gao |
| |
| Institution: | 1. Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China |
| |
| Abstract: | Murine leukemia virus (MLV)-based retroviral vectors is widely used for gene transfer and basic research, and production of high-titer retroviral vectors is very important. Here we report that expression of the Y-box binding protein 1 (YB-1) enhanced the production of infectious MLV vectors. YB-1 specifically increased the stability of viral genomic RNA in virus-producing cells, and thus increasing viral RNA levels in both producer cells and virion particles. The viral element responsive to YB-1 was mapped to the repeat sequence (R region) in MLV genomic RNA. These results identified YB-1 as a MLV mRNA stabilizer, which can be used for improving production of MLV vectors. |
| |
| Keywords: | Y-box binding protein 1 retroviral vector RNA stability |
|
| 点击此处可从《蛋白质与细胞》浏览原始摘要信息 |
| 点击此处可从《蛋白质与细胞》下载免费的PDF全文 |
|
