Effect of genotype,explant type and growth regulators on organogenesis in Morus alba |
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Authors: | Bhau BS Wakhlu AK |
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Institution: | (1) Plant Tissue Culture Laboratory, Department of Botany Jammu University, Jammu, 180 006 Jammu & Kashmir, India;(2) Plant Tissue Culture Laboratory, Department of Botany Jammu University, Jammu, 180 006 Jammu & Kashmir, India |
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Abstract: | Plantlets of the mulberry (Morus alba L. vars. Chinese White, and Kokuso-27) were produced from callus cultures. For callus induction, leaf, internodal segments,
and petiole explants of Chinese White, Kokuso-27 and Ichinose varieties were grown on MS basal medium fortified with 2,4-D
and 6-benzylaminopurine (BA). Callogenesis was dependent on the nature of explant used, the genotype and growth regulators
supplemented in the medium. Leaves were the best explant type used for callus induction. Best callogenesis was obtained on
MS medium containing a combination of 1 mg l−1 2,4-D and 0.5 mg l−1 BA (95-100%). Calluses formed shoots on MS medium supplemented with 1 mg l−1 BA. Supplementation with 0.1 mg l−1 2,3,5-triiodobenzoic acid (TIBA) in this medium enhanced shooting response. Presence of TIBA in the medium also improved
the long-term organogenic potential of the callus. Regenerated shoots produced roots on Murashige & Skoog (MS) medium containing
either 0.5 mg l−1 indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). Seventy percent of the rooted plants were established in the
field where they are performing well.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | auxin cytokinin genotype mature explant Morus mulberry organogenesis |
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