Killer cells of feline leukemia virus- and feline sarcoma virus-infected transformed cells: the role of NK, ADCC, and in vitro generated cytotoxic cells |
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| Authors: | L H Kooistra G A Splitter |
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| Affiliation: | 1. Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry,Marie Curie Building, Campus de Rabanales, University of Córdoba,14071, Córdoba, Spain;2. International Forensic Research Institute, Department of Chemistry and Biochemistry, Florida International University,11200 SW 8th Street, Miami, FL, 33199, USA;1. Neurovascular Research Group, Institute de Biomedicine of Seville, IBiS/Hospital Universitario Virgen del Rocío/CSIC/University of Seville, Av. Manuel Siurot s/n, 41013, Seville, Spain;2. Department of Neurology, Hospital Universitario Virgen Macarena, Avenida Doctor Fedriani, no. 3, 41007, Seville, Spain;1. Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad-500058, Telangana, India;2. School of Life Sciences, Department of Biotechnology and Bioinformatics, University of Hyderabad, Hyderabad-500046, Telangana, India;3. Department of Genetics, Osmania University, Hyderabad-500007, Telangana, India;1. Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA;2. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA 98109, USA;3. Department of Global Health, University of Washington, Seattle, WA 98195, USA;4. Department of Immunology & Microbiology, IAVI Neutralizing Antibody Center, Center for HIV/AIDS Vaccine Immunogen Development, The Scripps Research Institute, La Jolla, CA 92037, USA;5. Oregon National Primate Research Center, Oregon Health & Science University, Portland, OR 97006, USA;6. Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA;7. Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA;8. Beth Israel Deaconess Medical Center, Boston, MA 02115, USA;9. Department of Biostatistics, University of Washington, Seattle, WA 98195, USA;1. Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan;2. Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China;3. Department of Pediatrics, The Third Xiangya Hospital, Central South University, Changsha, China;4. AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan;5. SBI Pharmaceuticals Co., Ltd., Tokyo, Japan |
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| Abstract: | Feline white blood cells (WBC) manifested a primary in vitro mixed lymphocyte tumor cell culture (MLTC) proliferative response to feline leukemia virus-feline sarcoma virus (FeLV-FeSV)-infected transformed target cells, which reached a peak at Day 15. Furthermore, primary in vitro MLTC cultures generated cytotoxic killer cells capable of killing a variety of targets in non-major histocompatibility gene complex restricted fashion, and effector cells were capable of killing targets introduced repeatedly into cultures over a 49-day period. The presence of feline fibrosarcoma (f-sarc) stimulators was the primary driving force for proliferation and generation of killing because exogenous IL-2 conditioned medium did not appreciably increase the yield of killer cells generated in vitro. WBC cultured without f-sarc stimulators with or without IL-2 supplementation also generated killer (K) cells but at a low level. The killer cell population was composed of approximately 50% lymphocytes and 50% monocytes. Cats had K cells functional in antibody-dependent cell-mediated cytotoxicity against FeLV-coated chicken red blood cells but not against any FeLV-FeSV-infected transformed targets tested. Natural killer (NK) cell activity to any targets tested was not found. Although no evidence was found for K or NK cell activity against FeLV-FeSV-infected transformed cells, feline WBC were readily able to generate killer cells in vitro and it is probable that this cell-mediated immune potential is functionally important in vivo. |
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