首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Plant regeneration from isolated microspores of linseed (Linum usitatissimum L.)
Authors:Karin Nichterlein  Wolfgang Friedt
Institution:(1) Institute of Agronomy and Plant Breeding I, Justus-Liebig-University Giessen, Ludwigstr., 23, W-6300 Giessen, Germany;(2) Present address: School of Agricultural Sciences, University of Zambia, P.O. Box 32 379, Lusaka, Zambia
Abstract:Embryogenesis and plant regeneration were induced in isolated microspore culture of linseed (oilflax, Linum usitatissimum). Microspores underwent cell divison which led to either microcallus or embryoid formation, when they were cultured in a modified liquid Nitsch-Lichter-Nitsch medium (Lichter 1985) at two different incubation temperatures (30 and 35 °C); some embryoids and microcalli further developed to larger calli. After transfer of the microspore derived calli to a solid medium containing zeatin (Img 1–1) shoot induction was achieved from 36 to 66% of the calli. The highest frequency of regenerated plants was obtained in microspore cultures of the hybrid lsquoAtalantersquo x lsquoSzegedi 62rsquo (F1) at 30 °C, whereas for the second genotype lsquoPedigree 2rsquo x lsquoKiszombori 41rsquo (F2) the higher incubation temperature seemed to be more efficient. Shoots could be successfully rooted on an indole acetic acid containing medium and then transplanted to vermiculite and finally to soil. Most of the plants survived the transfer into soil in the greenhouse, where they could be successfully grown to maturity.Abbreviations BAP 6-benzylaminopurine - 2,4D dichlorophenoxyacetic acid - IAA indole acetic acid - N6 Chu (1978) medium - NAA naphthaleneacetic acid - NLN Nitsch-Lichter-Nitsch (1985) - MS Murashige and Skoog (1962) medium - ZEA zeatin
Keywords:Linseed  Microspore culture  Haploids  Plant regeneration
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号