Cell wall carbohydrates in Phycomyces blakesleeanus burgeff |
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Authors: | A J Van Laere A R Carlier J A Van Assche |
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Institution: | (1) Laboratorium voor Plantenbiochemie, K. U. Leuven, Vaartstraat 24, B-3000 Leuven, Belgium |
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Abstract: | The carbohydrate composition of the cell walls from spores, mycelium and sporangiophores of Phycomyces blakesleeanus was analyzed. Spore wall polysaccharides contained over 50% glucose, about 20% uronic acids, 10% mannose and 10% amino-sugars. During the growth of the hyphae amino-sugars became the main carbohydrate (45%); uronic acids contributed some 25%, glucose and fucose 10% and galactose nearly 6%. Sporangiophores contained almost 90% aminosugars and some 6% uronic acids. Traces of rhamnose were found in all wall preparations. A similar picture emerged from studies on the incorporation of U-14C]-glucose into wall materials.Furthermore we looked for a GDP-fucose synthesizing system and found an increasing activity during early germination. This rise in activity was inhibited by cycloheximide but not by 5-fluorouracil. |
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Keywords: | Phycomyces Cell wall carbohydrates GDP-fucose synthesis Spores Sporangiophores Hyphae |
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