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An improved system for the generation and analysis of mutant proteins containing unnatural amino acids in Saccharomyces cerevisiae |
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| Authors: | Chen Shawn Schultz Peter G Brock Ansgar |
| Institution: | Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. |
| Abstract: | |
| Keywords: | wt wild-type hSOD-His6 human superoxide dismutase with a six-histidine tag aaRS aminoacyl-tRNA synthetase pPpa p-propargyloxyphenylalanine pMpa p-methoxyphenylalanine pAzpa p-azidophenylalanine pApa p-acetylphenylalanine pBpa p-benzoylphenylalanine MRM multiple-reaction monitoring ESI electrospray ionization TOF time-of-flight MALDI matrix-assisted laser desorption/ionization LC liquid chromatography MS mass spectrometry |
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