Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12 |
| |
Authors: | P Broda P Meacock and M Achtman |
| |
Institution: | (1) MRC Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, Scotland;(2) MPI für Biologie, Corrensstr. 38, D-7400 Tübingen, W. Germany;(3) Department of Genetics, University of Leicester, Leicester, England |
| |
Abstract: | Summary Sixty-eight Hfr strains were examined for their ability to transfer early in conjugation the transfer genes carried by the integrated sex factor. This was measured by mating these strains with F- phenocopied recipient cultures of strains carrying transfer-deficient Flac
+ factors, and then measuring the ability of the recipient strains to transfer lac
+ to a further recipient strain. Most Hfr strains did not complement the missing transfer functions, though in some strains complementation was observed. It is concluded that on the sex factors of different Hfr strains either the site at which integration occurs or the origin of transfer must vary. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|