Purification of the autotransporter protein Hbp of Escherichia coli |
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Authors: | Silvy JM van Dooren Jeremy RH Tame Joen Luirink Bauke Oudega Ben R Otto |
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Institution: | Department of Molecular Microbiology, Institute of Molecular Biological Sciences, De Boelelaan 1087, 1081 HV Amsterdam, Netherlands. |
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Abstract: | The enzyme Hbp (hemoglobin protease) of the pathogenic Escherichia coli strain EB1 has been purified to homogeneity by gel filtration chromatography. The purified protein is capable of binding heme and shows hemoglobin protease activity. Our method of purification is applicable not only to Hbp but also to other autotransporter proteins and will contribute to a better understanding of the function-structure relationship of this family of proteins. |
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Keywords: | Autotransporter Hemoglobinase Heme uptake Virulence Escherichia coli |
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