The induction of murine tumor infiltrating lymphocytes (TIL) by interleukin-2 or T cell growth factor |
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Authors: | Norimichi Kan Keiichi Mise Masaki Nakanishi Takashi Okino Takehisa Harada You Ichinose Yoshio Moriguchi Tomoharu Sugie Li Li Masayuki Imamura |
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Institution: | (1) Laboratory of Oncologic Surgery, First Department of Surgery, Kyoto University, Faculty of Medicine, 54 Kawaracho, Shogoin, Sakyo-ku, 606 Kyoto, Japan |
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Abstract: | Mice were injected in the foot pad with either 5×105 syngeneic plasmacytoma (MOPC104E) or fibrosarcoma cells (Meth A). Lymph nodes containing tumor cells were harvested 14 days later and cultured. In the presence of recombinant interleukin-2 (r-IL-2) predominantly tumor cells proliferated. Culture with T cell growth factor (TCGF) resulted in the growth of lymphoid cells. Concanavalin A (Con A) had only a modest effect on elimination of tumor cells in the culture. Tumor-infiltrating lymphocytes (TIL) prepared from the lymph nodes showed specific tumor-neutralizing activity when grown in the presence of TCGF. In vitro examination revealed that Meth A cells could not be lysed by TIL, while TIL from MOPC tumors showed tumor specific activity. This study may explain negative results in human trials with TIL induced by IL-2 alone.Abbreviations r
recombinant
- IL-2
interleukin-2
- TCGF
T cell growth factor
- TIL
tumor infiltrating lymphocytes
- Con A
concanavalin A
- HBSS
Hanks' balanced salt solution |
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Keywords: | cytokine interleukin-2 killer cell T cell growth factor tumor infiltrating lymphocytes |
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