Evidence for Distinct Aggregation Factors and Receptors in Cells |
| |
Authors: | BERNARD PESSAC VITTORIO DEFENDI |
| |
Institution: | 1.The Wistar Institute of Anatomy and Biology,Philadelphia;2.Institut d'Immunobiologie, H?pital Broussais,Paris |
| |
Abstract: | SINCE the original observations by Wilson1 that dissociated sponge cells could reassociate in vitro, cell aggregation (or reaggregation) has been widely used as an operational criterion for the study of intercellular adhesion2. The introduction of rotation-mediated methods to promote cell aggregation3,4 led to the possibility of obtaining reproducible quantitative data. In these methods, suspensions of dissociated single cells are shaken under defined conditions of speed and temperature and cell aggregation is measured by either the size of aggregates or the number of single cells. The aggregation of dissociated cells from sponges5, chick and mouse embryos4 and tissue culture cells6 has been investigated with this method. Cells maintained in vitro seemed particularly suitable for studying mechanisms of cell aggregation as they represent a histotypically homogeneous population. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|