Organization and structure of Volvox beta-tubulin genes |
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Authors: | Jeffrey F Harper and Wolfgang Mages |
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Institution: | (1) Department of Biology, Washington University, 63130 St. Louis, MO, USA;(2) Lehrstuhl für Genetik, Universität Regensburg, D-8400 Regensburg, Federal Republic of Germany;(3) Present address: Department of Horticulture, University of Wisconsin, 53706 Madison, WI, USA |
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Abstract: | Summary Genomic clones encoding two Volvox -tubulin genes have been isolated and shown to represent the only two -tubulin genes in the genome. Restriction fragment length polymorphism analysis was used to demonstrate that the two genes are genetically linked. One of these genes was sequenced and the mRNA start site(s) determined by primer extension. A comparison of its sequence to those of the two -tubulin genes of Chlamydomonas revealed: (1) a high degree of conservation of the coding region, with the predicted amino acid sequence differing only in the C-terminal residue; (2) extensive sequence conservation in the 5 untranslated leader region and a 16 bp (putative regulatory) sequence in the promoter region; (3) the same number and location of introns, with a short region of homology in intron 1, but little significant homology in introns 2 and 3. |
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Keywords: | -Tubulin" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-Tubulin Codon usage DNA sequence Restriction fragment length polymorphism mapping Volvox |
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