| 热水提取酿酒酵母中S-腺苷-L-甲硫氨酸的研究 |
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| 引用本文： | 姚进孝,刘沛溢,谭天伟. 热水提取酿酒酵母中S-腺苷-L-甲硫氨酸的研究[J]. 生物加工过程, 2008, 6(1): 74-77 |
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| 作者姓名： | 姚进孝 刘沛溢 谭天伟 |
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| 作者单位： | 北京化工大学,生命科学与技术学院,北京市生物加工过程重点实验室,北京,100029 |
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| 基金项目： | 国家自然科学基金 , 北京市自然科学基金 , 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 研究了采用热水法直接提取酿酒酵母胞内产物S-腺苷-L-甲硫氨酸（SAM）北京，并且通过实验对影响SAM抽提的5个条件：抽提温度、抽提时间、热水用量、搅拌转速、硫酸用量等进行了优化。得出的最佳实验条件为：温度70℃、每30g湿菌体加入热水100mL、硫酸浓度0．25mol／L、搅拌转速160r／min，此时SAM的抽提率可达91．5％。与其他方法相比，该方法耗时少、仪器简单、抽提液中S-腺苷-L-甲硫氨酸质量浓度高、经济且无污染。
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| 关 键 词： | S-腺苷-L-甲硫氨酸 提取 酿酒酵母 |
| 文章编号： | 1672-3678（2008）01-0074-04 |
| 收稿时间： | 2007-04-10 |
| 修稿时间： | 2007-04-10 |
Using hot water to extract S -adenosyl- L -methionine from Saccharomyces cerevisiae |
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| YAO Jin-xiao,LIU Pei-yi,TAN Tian-wei. Using hot water to extract S -adenosyl- L -methionine from Saccharomyces cerevisiae[J]. Chinese Journal of Bioprocess Engineering, 2008, 6(1): 74-77 |
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| Authors: | YAO Jin-xiao LIU Pei-yi TAN Tian-wei |
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| Affiliation: | (Key Lab of Bioprocess of Beijing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China) |
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| Abstract: | A method for extracting S-adenosyl-L-methionine from Saccharomyces cereuisiae with hot water was investigated. Experimental results showed the optimum condition was at temperature of 70℃, the ra- tio of hot water to wet yeast at 100 mL： 30 g, the concentration of sulfuric acid at 0. 25 mol/L, and rota- ting speed at 160 r/min. Extraction rate reached upto 91.5%. The hot water extraction process featured a convenient, fast, effective, economical and non-contaminated method for the extraction of S-adenosyl- L-methionine. |
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| Keywords: | S-adenosyl-L-methionine extraction Saccharomyces cerevisiae |
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