Long-term exposure of CdTe quantum dots on PC12 cellular activity and the determination of optimum non-toxic concentrations for biological use |
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| Authors: | Babu R Prasad Natalia Nikolskaya David Connolly Terry J Smith Stephen J Byrne Valérie A Gérard Yurii K Gun'ko and Yury Rochev |
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| Institution: | (1) National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland;(2) CRANN and The School of Chemistry, Trinity College Dublin, Dublin 2, Ireland |
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| Abstract: | Background The unique and tuneable photonic properties of Quantum Dots (QDs) have made them potentially useful tools for imaging biological
entities. However, QDs though attractive diagnostic and therapeutic tools, have a major disadvantage due to their inherent
cytotoxic nature. The cellular interaction, uptake and resultant toxic influence of CdTe QDs (gelatinised and non-gelatinised
Thioglycolic acid (TGA) capped) have been investigated with pheochromocytoma 12 (PC12) cells. In conjunction to their analysis
by confocal microscopy, the QD - cell interplay was explored as the QD concentrations were varied over extended (up to 72
hours) co-incubation times. Coupled to this investigation, cell viability, DNA quantification and cell proliferation assays
were also performed to compare and contrast the various factors leading to cell stress and ultimately death. |
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