Probiotic lactic acid bacterium from <Emphasis Type=Italic>kanjika</Emphasis> as a potential source of vitamin B<Subscript>12</Subscript>: evidence from LC-MS,immunological and microbiological techniques期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC-MS,immunological and microbiological techniques

Authors:	Arenahalli Ningegowda Madhu Prashant Giribhattanavar Mandayam Singara Narayan Siddalingaiya Gurudutt Prapulla

Affiliation:	(1) Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, 570 020, India;(2) Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore, 570 020, India;

Abstract:	Vitamin B₁₂ was produced by probiotic Lactobacillus plantarum in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h each to give 13 ng vitamin B₁₂/g dry biomass. Sodium cyanide-mediated cell lysis, followed by benzyl alcohol/chloroform/water extraction, improved the release of intracellular vitamin B₁₂ for analysis. The presence of the K⁺ adduct of cyanocobalamin (m/z of 1394) was established using electron spray ionization–mass spectra; growth of a mutant of Escherichia coli in the presence of cyanocobalamin ascertained its bioavailability.

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