In vivo gene expression: combining hydrodynamics-based transfection and electrotransfer

Clinical development of gene therapy is hampered by the lack of an efficient and safe method for in vivo gene transfer. New methodologies for plasmid transfer are being developed. Recently, significant expression of a reporter gene was achieved in liver cells by coupling intravenous injection and stimulation of the tissue with electric pulses. This combination of hydrodynamics-based transfection and electrotransfer could provide the basis for many promising clinical applications.