Synthesis of new steroid haptens for radioimmunoassay. Part II. 15beta-Carboxyethylmercaptodehydroepiandrosterone bovine serum albumin conjugate. Specific antiserum for solid-phase radioimmunoassay of dehydroepiandrosterone. |
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Authors: | P Narasimha Rao P H Moore |
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Institution: | Southwest Foundation for Research and Education P.O. Box 28147, 8848 West Commerce Street, San Antonio, Texas 78284 USA |
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Abstract: | Antiserum for radioimmunoassay (RIA) of dehydroepiandrosterone (DHA) was raised in rabbits with the conjugate obtained by coupling 15beta-carboxyethylmercapto-dehydroepiandrosterone with bovine serum albumin. The antiserum proved to have high affinity and specificity for DHA and showed only minor cross-reaction to androst-5-ene-3beta, 17beta-diol (3.5%). The Rivanol-treated antiserum was covalently coupled to Enzacryl Polyacetal to evaluate its utility in solid-phase RIA. |
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Keywords: | Etiocholanolone 3α-hydroxy-5β-androstan-17-one Estriol 1 3 5(10)-estratrinen-3 16α 17β-triol |
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