|
|||||
|
|
| 酒类酒球菌苹果酸-乳酸酶基因的克隆 | |
| 引用本文： | 张春晖,夏双梅,刘天明. 酒类酒球菌苹果酸-乳酸酶基因的克隆[J]. 微生物学杂志, 2003, 23(3): 21-23 |
| 作者姓名： | 张春晖 夏双梅 刘天明 |
| 作者单位： | 1. 双汇集团-江南大学食品科学与工程博士后工作站,河南,漯河,462000 2. 信阳农业高等专科学,校植物科学系,河南,信阳,464000 3. 华中农业大学,生命科学学院,湖北,武汉,430070 |
| 基金项目： | 河南省杰出青年科学基金资助项目(0312002800) |
| 摘 要： | 利用PCR方法从酒类酒球菌(Oenococcus oeni)基因组中扩增出651 bp的DNA片段,将之克隆到pUC19-T载体上并转化大肠杆菌(E.coli)JM109菌株.重组质粒的测序结果表明,克隆到了苹果酸-乳酸酶基因(mle),它含有527 bp的阅读框架,其核苷酸序列与文献报道相同. |
| 关 键 词： | 酒类酒球菌 基因克隆 苹果酸-乳酸酶基因(mle) |
| 文章编号： | 1005-7021(2003)03-0021-03 |
| 修稿时间： | 2002-10-28 |
Malolactic Enzyme Gene (mle) Cloning from Oenococcus oeni |
|
| ZHANG Chunhui+,XIA Shuangmei+,LIU Tianming+. Malolactic Enzyme Gene (mle) Cloning from Oenococcus oeni[J]. Journal of Microbiology, 2003, 23(3): 21-23 | |
| Authors: | ZHANG Chunhui+ XIA Shuangmei+ LIU Tianming+ |
| Affiliation: | ZHANG Chunhui+1,XIA Shuangmei+2,LIU Tianming+3 |
| Abstract: | A DNA fragment about 530bps was amplified from geneme DNA of Oenococcus oeni using PCR technique. The fragment was then cloned into pCU19 vector and subsequently transformed into E.coli JM109. The sequencing result demonstrated that the cloned DNA fragment was malolactic enzyme gene(mle). It is an ORF of 527bp including signal peptide enconding region, and it has the same sequence as described in literature.; |
| Keywords: | Oenococcus oeni gene cloning malolactic enzyme gene(mle) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |