| 抗生素AGPM发酵过程中蛋白质表达差异的研究 |
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| 引用本文： | 牛晋阳,乔建军,陈贵斌,张建勇,元英进. 抗生素AGPM发酵过程中蛋白质表达差异的研究[J]. 微生物学通报, 2003, 30(3): 6-9 |
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| 作者姓名： | 牛晋阳 乔建军 陈贵斌 张建勇 元英进 |
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| 作者单位： | 天津大学化工学院,天津,300072 |
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| 基金项目： | 国家自然科学基金资助项目 (No 3 0 2 0 0 0 0 3 )，国家高技术研究发展计划项目 (“863”项目 ) (No 2 0 0 1AA2 14 0 81)~~ |
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| 摘 要： | 由土壤中筛选到一能产生新型抗肿瘤抗生素AGPM的藤黄灰链霉菌株，应用蛋白质双向电泳方法，比较了藤黄灰链霉菌在发酵24h与72h蛋白质表达的差异，发现在发酵72h有17个蛋白质差异点出现，此外还发现一些蛋白质的含量明显高于24h的同种蛋白含量，这表明这些蛋白可能与抗生素AGPM的合成以及和菌体的生长等有关。
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| 关 键 词： | 藤黄灰链霉菌 AGPM 蛋白质组 二维凝胶电泳 |
| 文章编号： | 0253-2654(2003)03-0006-04 |
| 修稿时间： | 2002-06-04 |
THE STUDY ON DIFFERENCE OF PROTEIN EXPRESSIONS IN AN ANTIBIOTIC AGPM FERMENTATION PROCESS |
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| NIU Jin Yang QIAO Jian Jun CHEN Gui Bin ZHANG Jian Yong YUAN Ying Jin. THE STUDY ON DIFFERENCE OF PROTEIN EXPRESSIONS IN AN ANTIBIOTIC AGPM FERMENTATION PROCESS[J]. Microbiology China, 2003, 30(3): 6-9 |
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| Authors: | NIU Jin Yang QIAO Jian Jun CHEN Gui Bin ZHANG Jian Yong YUAN Ying Jin |
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| Abstract: | A new strain of Streptomyces regensis was isolated from soil to produce a novel antibiotic AGPM possessing a strong antitumor activity In order to study on the metabolic path of the novel antibiotic AGPM, the protein patterns from the strain of Streptomyces regensis at different culture period were analyzed by using two eimensional polyacrylamide gel electrophoresis Comparing with sample from growth phase, seventeen new protein spots were found in that from antibiotic production phase The results demonstrated that the special proteins might be related with the antibiotic AGPM biosynthesis from Streptomyces regensis |
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| Keywords: | Streptomyces regensis AGPM Proteome Two dimensional gel electrophoresis |
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