Involvement of cellulose synthesis in actions of gibberellin and kinetin on cell expansion. Gibberellin-coumarin and kinetin-coumarin interactions on stem elongation |
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Authors: | Hogetsu Taizo; Shibaoka Hiroh; Shimokoriyama Masami |
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Institution: | Department of Botany, Faculty of Science, University of Tokyo Hongo, Tokyo, Japan |
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Abstract: | In azuki bean (Azukia angularis = Vignia angularis) epicotylsections, 5 ? 104 M coumarin inhibited the incorporationof radioactivity from U14C]glucose into the cellulosefraction by 35% in the absence of indole-3-acetic acid (IAA)and by 40% in the presence of 1 ? 104 M IAA. There wasno inhibitory effect on the incorporation of radioactivity intothe other fractions. Coumarin at 5 ? 104 M reversed thepromoting effect of 1 ? 105 M gibberellin A3 (GA) andthe inhibitory effect of 1 ? 105 M kinetin on IAA-inducedelongation of sections with no significant effects on IAA-inducedelongation. Neither GA nor kinetin had any appreciable effectson cellulose synthesis. No inhibition of cellulose syntheiswas observed with 1 ? 103 M colchichine, which has beenreported to have effects similar to those of coumarin on GA-or kinetin-affected stem elongation. Coumarin at 5 ? 104M was ineffectual in breaking up wall microtubules, while adisrupting effect on wall microtubules was clearly demonstratedwith 3 ? 104M colchicine. From these results, the possible involvement of cellulose synthesisin cell expansion controlled by GA or kinetin was suggested. (Received August 3, 1973; ) |
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