Content, distribution and stability of protein-synthesis elongation factor Tu in subcellular fractions of vegetative cells and spores of Streptomyces aureofaciens |
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Authors: | J Weiser P Morava J Vohradský |
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Institution: | Institute of Microbiology, Czechoslovak Academy of Sciences, Prague. |
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Abstract: | The protein synthesis elongation factor Tu (EF-Tu) was identified in dormant spores of Streptomyces aureofaciens and its content and distribution in vegetative cells and dormant spores were determined. Cell-free homogenates from spores were found to contain a EF-Tu cleaving membrane bound protease. The protease cleaved aggregated EF-Tu much less efficiently than non-aggregated factor in cell homogenates. The relative content of EF-Tu and ribosomes in dormant spores was very similar to that found in exponentially growing vegetative cells. |
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Keywords: | Streptomyces aureofaciens Protein synthesis Vegetative cells |
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