Effects of Chromium Picolinate on Micronucleus Frequency and Morphology of Lymphocytes in Calves |
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Authors: | Nalan İmamoğlu Fatma Uyanık Berrin Kocaoğlu Güçlü Onur Erdem Bilal Cem Liman Hamiyet Dönmez Altuntaş |
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Institution: | Halil Bayraktar Health Service Vocational College, University of Erciyes, Kayseri, Turkey. |
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Abstract: | We report the effects of chromium picolinate (CrPic) on micronucleus frequency, morphology of lymphocytes, and lipid peroxidation
in calves. Twenty-four Holstein calves were selected for the study. They were kept in a farm and were fed a commercially available
calf diet and alfalfa, ad libitum. The animals were divided into three groups of eight subjects each and were treated as follows:
The first group was supplemented with a daily dose of 200 μg Cr as chromium picolinate; a second group received 400 μg Cr
per day and a third group that served as control received no supplemental chromium. After 12-week supplementation, blood samples
were collected to determine the micronucleus frequency, the apoptotic cell percentage, and the malondialdehyde (MDA) and blood
chromium levels. In both supplemented groups, the cells had irregularly shaped and segmented nuclei. Supplementation also
increased the percentage of apoptotic cells (p < 0.001) and serum MDA (p < 0.01) and slightly increased the chromium levels. The animals supplemented with 400 μg showed a significant increase of
micronucleus frequency (p < 0.01). The results of this study suggest that supplementation with 200 and 400 μg chromium as chromium picolinate may lead
to cytotoxicity. The higher level of supplementation may also have genotoxic effects. However, further studies investigating
the mechanism of the action of CrPic are required. |
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Keywords: | Calves Chromium picolinate Cytotoxicity Genotoxicity Lipid peroxidation |
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