Protection against malaria induced by chirally modified Plasmodium falciparum's MSP-1 42 pseudopeptides |
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Authors: | Lozano José Manuel Espejo Fabiola Vera Ricardo Vargas Luis Eduardo Rosas Jaiver Lesmes Liliana Torres Elizabeth Cortés Jimena Silva Yolanda Patarroyo Manuel Elkin |
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Affiliation: | Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D.C., Colombia. jm_lozano@fidic.org.co |
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Abstract: | The C-terminal portion of the Plasmodium falciparum blood stage MSP-1 antigen plays a key role in invasion of human erythrocytes. The MSP-1(1282-1301) non-polymorphic 1585 peptide, from the processed MSP-1(42) fragment, is poorly immunogenic and highly alpha-helical [Angew. Chem. Int. Ed. 40 (2001) 4654]. Assessing the alpha-carbon asymmetry and its implication in the host immune response is proposed in this work to overcome the 1585 peptide's immunological properties. Accordingly, the effect of incorporating single D-amino acids and psi-[CH(2)-NH] isoster bonds into the 1585 peptide was examined both at the immunogenic and 3D-structure levels. Therefore, specific binding to RBCs is promoted by site-directed chiral modifications on the native peptide as well as by simultaneously combining specific D-substitutions with psi-[CH(2)-NH] isoster bonds transforming this molecule into a high specific HLAbeta1*1101 allele binder. D-analog pseudopeptide immunized animals induced antibodies selectively recognizing a recombinant as well as native MSP-1(42) and MSP-1(33) fragments. Protection and low parasitemia levels were induced in Aotus monkeys immunized with the EVLYL(dK)PLAGVYRSLKKQLE analog. Peptide alpha-carbon chiral transformation is therefore an important target for structural modulation and, consequently, represents a novel approach towards designing multi-component subunit-based malarial vaccines. |
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Keywords: | Chirality α-carbon asymmetry Merozoite surface protein-1 Pseudopeptide Sub-unit malarial vaccine |
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