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PCR screening and sequence analysis of |
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| Authors: | W. Zhang Z. Sun T. Sun H. Zhang |
| Affiliation: | (1) Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, School of Food Science and Engineering, Inner Mongolia Agricultural University, 010018 Huhhot, China;(2) School of Agriculture and Biology, Shanghai Jiao Tong University, 200240 Shanghai, China;(3) Beijing Institute of Genomics and Key Laboratory of Genome Science and Information, Chinese Academy of Sciences, 100029 Beijing, China;(4) James D. Watson Institute of Genome Sciences, Zhejiang University, 310029 Hangzhou, China;(5) School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China; |
| Abstract: | The iol cluster (consisting of genes involved in myo-inositol utilization) was investigated in Lactobacillus casei strains isolated from koumiss. Ten strains were tested for the presence of iol cluster by PCR screening; three strains encoded this cluster. Full-sequencing procedure was conducted; the iol cluster was identical to that of L. casei BL23 (GenBank access. no. FM177140) except for an upstream transposase. The iol cluster is not a common feature for L. casei strains isolated from koumiss. |
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