Genetic Relationship of <Emphasis Type="Italic">Curcuma</Emphasis> Species from Northeast India Using PCR-Based Markers |
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Authors: | Archana Das Vigya Kesari Vinod M Satyanarayana Ajay Parida Latha Rangan |
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Institution: | (1) Department of Biotechnology, Indian Institute of Technology Guwahati, North Guwahati, 781 039, Assam, India;(2) M S Swaminathan Research Foundation, 3rd Cross Street, Taramani Institutional Area, Chennai, 600 113, India; |
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Abstract: | Molecular genetic fingerprints of nine Curcuma species from Northeast India were developed using PCR-based markers. The aim involves elucidating there intra- and inter-specific
genetic diversity important for utilization, management, and conservation. Twelve random amplified polymorphic DNA (RAPD),
19 Inter simple sequence repeats (ISSRs), and four amplified fragment length polymorphism (AFLP) primers produced 266 polymorphic
fragments. ISSR confirmed maximum polymorphism of 98.55% whereas RAPD and AFLP showed 93.22 and 97.27%, respectively. Marker
index and polymorphic information content varied in the range of 8.64–48.1, 19.75–48.14, and 25–28 and 0.17–0.48, 0.19–0.48,
and 0.25–0.29 for RAPD, ISSR, and AFLP markers, respectively. The average value of number of observed alleles, number of effective
alleles, mean Nei’s gene diversity, and Shannon’s information index were 1.93–1.98, 1.37–1.62, 0.23–0.36, and 0.38–0.50, respectively,
for three DNA markers used. Dendrograms based on three molecular data using unweighted pair group method with arithmetic mean
(UPGMA) was congruent and classified the Curcuma species into two major clusters. Cophenetic correlation coefficient between dendrogram and original similarity matrix were
significant for RAPD (r = 0.96), ISSR (r = 0.94), and AFLP (r = 0.97). Clustering was further supported by principle coordinate analysis. High genetic polymorphism documented is significant
for conservation and further improvement of Curcuma species. |
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